Hanges inside the number (cfu mL-1 ) of bacteria had been observed at the end on the assay (information not shown). Employing these results, the 18 Azotobacter strains were arbitrarily classified as low- (2? g mL-1 ), medium- (7?14 g mL-1 ), and high- (14 g mL-1 ) auxin producers (Table 2). Then, we selected 3 low-auxin-producing strains (AT18, AT37, and AT42) and 3 high-auxinproducing strains (AT19, AT25, and AT31) and assessed them in nitrogen fixing capacity and biosynthesis of three phytohormones (IAA, GA3 , and Z). Concerning the nitrogenase activity, the highest activity levels (14 mmol C2 H4 mg protein-1 24 h-1 ) have been exhibited by A. salinestris AT42 in addition to a. chroccoccum AT31 strains.The Scientific World JournalTable 2: Auxin production capacity of representative Azotobacter strains as determined by a colorimetric assay using the Salkowski reagent and benefits of rep-PCR clustering. Strain A. chroococcum AT9 A. chroococcum AT11 A. chroococcum AT13 A. chroococcum AT22 A. chroococcum AT31 A. chroococcum AT28 A. chroococcum AT25 A. chroococcum AT39 A. chroococcum AT30 A. armeniacus AT33 A. salinestris AT18 A. salinestris AT19 A. salinestris AT14 A. salinestris AT42 A. salinestris AT29 A. salinestris AT37 A. salinestris AT12 A. salinestris AT10 rep-PCR group 1 1 1 1 1 1 1 1 1 2 three three three 4 four 5 5 6 Auxin production (g mL-1 ) Day 2 Day 3 2.50 ?0.27a 4.13 ?0.30c four.97 ?1.27a 12.26 ?0.25a four.58 ?1.37a 11.38 ?3.52a a eight.54 ?two.02 12.62 ?0.61a a 9.30 ?0.60 12.68 ?1.65a a 7.81 ?0.44 14.80 ?0.10a a 7.97 ?2.31 12.82 ?0.07a a six.01 ?1.09 12.92 ?1.40a a 4.97 ?1.27 12.86 ?0.60a a five.95 ?0.29 9.86 ?0.45b a 2.09 ?1.08 3.42 ?0.21c 6.09 ?0.43a 15.38 ?0.68a a 3.41 ?0.69 12.38 ?0.37a a three.64 ?0.30 5.14 ?0.76c a 3.32 ?0.60 7.21 ?0.40b a 1.82 ?0.08 five.22 ?1.28c a 2.91 ?0.43 eight.23 ?0.12b a three.20 ?0.72 five.51 ?0.15cDay 1 1.80 ?0.64a 1.ten ?0.41a 1.41 ?0.83a 2.08 ?0.09a 1.38 ?0.61a 2.16 ?0.81a two.64 ?0.57a 1.59 ?0.40a 1.40 ?0.60a 1.92 ?0.32a 1.72 ?0.27a 1.34 ?0.09a two.15 ?0.08a 1.90 ?0.33a 1.24 ?0.76a 0.96 ?0.55a 1.45 ?0.60a 1.38 ?0.13aDay five 4.23 ?1.55c 12.34 ?two.07b 12.72 ?2.46b 13.25 ?1.54b 14.76 ?0.52b 14.81 ?0.93b 15.33 ?two.41b 15.36 ?0.03b 16.11 ?0.44b 9.69 ?1.06b 2.22 ?1.59c 18.09 ?0.98a 19.47 ?0.48a five.93 ?1.69c ten.62 ?1.73b 6.25 ?0.08c 13.59 ?1.30b six.58 ?2.38cAuxin concentrations had been determined right after 1, 2, 3, and 5 days of growth in liquid culture. Values are means of two replicates ?common error ( = 2). Similar letters in a column indicate no substantial HSP90 Activator Storage & Stability differences as determined by the DGC test ( = 0.05).Table 3: Impact of pure IAA solutions and Azotobacter inoculation around the quantity of seminal roots, and root colonization of 8-day-old wheat seedlings. Bax Activator Storage & Stability Therapy Water Low-IAA High-IAA A. salinestris AT18 A. salinestris AT37 A. chroococcum AT25 A. chroococcum AT31 A. salinestris AT19 IAA concentration g mL-1 2 20 two.two 2.five 14.eight 14.2 18.2 Number of seminal roots per plant four.33 ?0.12c 4.69 ?0.11b five.03 ?0.12a 4.68 ?0.10b 4.74 ?0.16b 5.18 ?0.18a 5.33 ?0.15a 5.39 ?0.06a Root colonization (cfu root-1 ) — — — c 4.38 ?104 ?1.68 ?104 b six.94 ?105 ?2.67 ?105 a 1.26 ?106 ?4.67 ?105 b 8.41 ?105 ?1.87 ?105 a three.01 ?106 ?1.12 ?Pregerminated seeds had been treated with water (Control), IAA pure solutions of two g mL-1 (Low-IAA) and 20 g mL-1 (High-IAA), or were inoculated using a. salinestris strains (AT18, AT19, and AT37) or a. chroococcum strains (AT25 and AT31). Values are indicates ?normal error of two independent experiments with 3 replicates ( = six). Different letters inside a column indicate significan.