ly unique involving the high-yielding strain (H) and also the low-yielding strain (L) in the 3 modules of blue, brown, and bisque4. The outcomes of correlation analysis involving two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are drastically negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, with a correlation coefficient of 0.24. GO and KEGG enrichment evaluation on blue, brown, and bisque4.GO enrichment evaluation was carried out on genes inside the 3 modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The outcomes showed that genes in these 3 modules were primarily enriched in catalytic activity and binding within the molecular functions; metabolic processes, cellular processes, and single-organism RSK1 Species processes in the biological processes; and cell and cell parts in the cellular element. The 3 modules had precisely the same GO enrichment final results, only the number of genes was distinct. Furthermore, KEGG enrichment benefits (Supplementary Table S3) for the 3 modules had been not the same. The brown module (P 0.05) was primarily enriched within the metabolic pathways of glyceride, sulfur, and galactose; non-homologous finish joining; and microbial metabolism in diverse environments (Fig. three). The blue module (P 0.05) was primarily enriched inside the metabolism and biosynthesis of numerous amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs throughout 3 SSTR3 custom synthesis cultivation periods of high-yielding strains; (b) Venn diagram of DEGs through three cultivation periods with the low-yielding strains; (c) Venn diagram of DEGs between high-yielding and low-yielding strains in 3 culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, between high- and low-yielding strains. transcription variables, etc. (Fig. four). The bisque4 module (P 0.05) was mostly enriched within the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. five). The KEGG enrichment final results of the 3 modules have been significantly different, which was consistent with all the benefits of the module correlation analysis. The genes related to triterpenoid anabolism in each and every module had been selected according to KEGG annotation benefits of genes, and these genes using the above gene’s expression correlation weight value among inside the module have been top rated ten have been chosen (Supplementary Table S4). These genes have been selected for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these chosen genes had been mostly concentrated in catalytic activity and binding inside the molecular functions; metabolic processes, cellular processes, and single-organism processes inside the biological processes; and cell and cell components in the cellular element. The enrichment of these three modules’ genes was nevertheless fundamentally the same. Detailed GO information and facts of those 3 modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment results of genes associated with triterpenoid biosynthesis in every single module (Supplementary Table S8