eolin, andFrontiers in Veterinary Science | frontiersin.orgOctober 2021 | Volume 8 | ArticleZhang et al.Anti-diarrhea Mechanism Evaluation of QJCFIGURE 8 | The morphological and histopathological changes (H E, 0 magnification) with the duodenum tissues in diarrhea mice. (A) Regular handle (NC) group, (B) model manage (MC) group, (C) optimistic control (Computer) group, (D) QJC group, (E) quercetin group, (F) kaempferol group, (G) luteolin group, (H) scutellarein group, and (I) stigmasterol group. Yellow arrow: exfoliated mucosal epithelial cells; blue arrow: intestinal crypts; green arrow: goblet cells; red arrow: loose connective tissue; n = five per group.stigmasterol groups could evidently depress the abscission of epithelial cells with the ileal mucosa and showed that the number of goblet cells plus the parameters of villus height/crypt depth had been superior compared with those of the MC group.DISCUSSIONNotably, TCM has complex components, producing it tough to elucidate its particular mechanisms. Simultaneously, both network pharmacology and TCM have the qualities of becoming multicomponent, multi-target, and multi-pathway approaches and are widely implemented to investigate the correlation among biological systems, drugs, and ailments (32). Weaning is one of the crux stages in pig production; the period is generally accompanied by diarrhea, which incurs excellent economic losses (two). Preliminary clinical studies in our laboratory have shown that QJC, which is composed of A. propinquus Schischkin (HQ), Z. HDAC8 web officinale Roscoe (SJ), and P. asiatica L. (CQC), has an effective anti-diarrhetic effect in vivo. Within this study, the chemical elements of QJC inside the therapy of diarrhea have been studied by means of the ADME evaluation and topological analysis to predict the core compounds responsible for treating diarrhea. The outcomes revealed quercetin, luteolin, kaempferol, scutellarein, and isorhamnetin as the key active compounds. Earlier studies have reported that quercetin has anti-diarrhea effects (33) and that the underlying anti-diarrhea mechanism is linked with enhancing the epithelial barrier (34). Our preceding study has revealed luteolin and scutellarein because the main anti-diarrhea components of CQC. They could improve the content material of Na+ and K+ by upregulating the activity and gene level ofEffects of QJC on the PI3K kt Signaling Pathway in Serotonin Hydrochloride-Infected MiceQuantitative real-time PCR (RT-qPCR) final results (Figure 11) showed that within the duodenum, jejunum, and ileum, compared with the NC group, the MC group relative expression levels of PI3K and Akt were substantially decreased (p 0.05), and the relative expression levels of PTEN had been significantly improved (p 0.05). Additionally, compared using the MC group, the expression levels of PI3K and Akt have been increased in every single drug therapy group (p 0.05), whereas the expression levels of PTEN were decreased (p 0.05). Nonetheless, these indices mentioned above could not be entirely restored to regular levels within the drug remedy groups. Meanwhile, we found that the therapeutic effect of QJC and stigmasterol to treat CXCR1 list tension diarrhea may possibly be consistent with loperamide hydrochloride (p 0.05).Frontiers in Veterinary Science | frontiersin.orgOctober 2021 | Volume eight | ArticleZhang et al.Anti-diarrhea Mechanism Analysis of QJCFIGURE 9 | The morphological and histopathological adjustments (H E, 0 magnification) of the jejunum tissues in diarrhea mice. (A) Regular manage (NC) group, (B) model handle (MC) group, (C)