Y studies. Based on the HepG2 and HepG2-CYP3A4 inC.
Y research. Depending on the HepG2 and HepG2-CYP3A4 inC. Schulz et al. / Inhibition of phase-1 biotransformation and cytostatic effects of diphenyleneiodoniumvitro model systems used, the outcomes show that DPI mediated inhibition of phase-1 biotransformation may be achieved. DPI is usually utilised as an inhibitor of CYP3A4 activity at concentrations as much as 50 nM without having inducing any morphological or toxic effects on the cells. At concentrations 50 nM, cytostatic effects on HepG2 or HepG2-3A4 are to become expected, so that influences or interactions with activity determinations can not be excluded, which have to be taken into account accordingly.Acknowledgments This work was funded by grants with the Ministerium fr Wirtschaft, Forschung und Kultur (MWFK, u state of Brandenburg, Germany) for the Fraunhofer Project Group “Pilzbasierte zellfreie SynthesePlattformen PZ-Syn” (project number 22-F241-03-FhG/005/001).
Journal ofFungiArticleWhole Genome Sequencing and Annotation of Naematelia aurantialba (Basidiomycota, Edible-Medicinal Fungi)Tao Sun 1 , Yixuan Zhang 1 , Hao Jiang 1 , Kai Yang 1 , Shiyu Wang 1 , Rui Wang 1 , Sha Li 1 , Peng Lei 1, , Hong Xu 1, , Yibin Qiu two and Dafeng SunState Key Laboratory of Materials-Oriented GLUT2 Biological Activity Chemical Engineering, College of Meals Science and Light Sector, Nanjing Tech University, Nanjing 211816, China; [email protected] (T.S.); [email protected] (Y.Z.); [email protected] (H.J.); [email protected] (K.Y.); [email protected] (S.W.); [email protected] (R.W.); [email protected] (S.L.) College of Light Market and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; [email protected] Kunming Edible Fungi Institute of All China Federation of Provide and Marketing Cooperatives, Kunming 650032, China; [email protected] Correspondence: [email protected] (P.L.); [email protected] (H.X.); Tel.: +86-187-6168-1790 (P.L.); Tel./Fax: +86-25-5813-9433 (H.X.)Citation: Sun, T.; Zhang, Y.; Jiang, H.; Yang, K.; Wang, S.; Wang, R.; Li, S.; Lei, P.; Xu, H.; Qiu, Y.; et al. Complete Genome Sequencing and Annotation of Naematelia aurantialba (Basidiomycota, Edible-Medicinal Fungi). J. Fungi 2022, 8, 6. doi/10.3390/jof8010006 Academic Editors: Luc Ram ez and Antonio Pisabarro Received: 11 November 2021 Accepted: 21 December 2021 Published: 22 December 2021 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Abstract: Naematelia aurantialba is a rare edible fungus with each nutritional and medicinal values and in particular rich in MAO-B Source bioactive polysaccharides. However, because of the lack of genomic facts, researches around the mining of active compounds, artificial breeding and cultivation, genetics, and molecular biology are limited. To facilitate the medicinal and food applications of N. aurantialba, we sequenced and analyzed the entire genome of N. aurantialba for the first time. The 21-Mb genome contained 15 contigs, as well as a total of 5860 protein-coding genes had been predicted. The genome sequence shows that 296 genes are related to polysaccharide synthesis, like 15 genes associated with nucleosideactivated sugar synthesis and 11 genes associated with glucan synthesis. The genome also includes genes and gene clusters for the synthesis of other active substances, like terpenoids, unsaturated fatty acids, and bioactive proteins. Moreover, it was also located that N. aurantialba was extra closely related to Naematelia encephala than to Tremella fuciformis. In quick, this.