Tabolism, signal transduction, amino acids, TGF-beta/Smad MedChemExpress tetrapyrrole binding, carboxypeptidase activity, amylase activity
Tabolism, signal transduction, amino acids, tetrapyrrole binding, carboxypeptidase activity, amylase activity, and cell cycle regulation have been upregulated. Carboxypeptidase can hydrolyze polypeptides into amino acids. Chlorophyll belongs towards the category of tetrapyrrole derivatives. Enrichment analysis of KEGG metabolic pathways (Fig. 2: g ) revealed that following BR spraying, the expression of protein processing-related genes within the endoplasmic reticulum was significantly upregulated. Protein processing in the endoplasmic reticulum incorporates glycosylation, hydroxylation, acylation, and disulfide bond formation, of which by far the most crucial is glycosylation. Practically all proteins synthesized inside the endoplasmic reticulum are lastly glycosylated. Genes connected to starch and sucrose metabolism had been drastically upregulated in CAC (BR spraying for 24 h). Genes related to ubiquitin-mediated proteolysis had been significantly upregulated in CAD (BRsJin et al. BMC Genomics(2022) 23:Page 7 ofFig. 2 a The number of differential genes up- or downregulated by the four comparison combinations (CAA vs. CAK, CAB vs. CAK, CAC vs. CAK, and CAD vs. CAK). b Venn diagram of 4 comparative combinations. c Column chart of GO enrichment analysis of upregulated differentially expressed genes in c CAA vs. CAK, d CAB vs. CAK, e CAC vs. CAK, and f CAD vs. CAK. g , g CAA vs. CAK upregulation within the bubble map of differentially expressed genes by KEGG enrichment evaluation. KEGG enrichment evaluation bubble chart of upregulated genes in h CAB vs. CAK, i CAC vs. CAK, and j KEG CAD vs. CAKsprayed for 48 h). Ubiquitin-mediated proteolysis produces amino acids. GO and KEGG enrichment analyses showed that just after spraying BRs onto tea leaves, genes related to sugar, starch, chlorophyll metabolism, the cell cycle, signal transduction, and amino acid Trk Receptor Source synthesis were upregulated.qRT-PCR evaluation of DEGsTo confirm the gene expression patterns detected around the transcriptome dataset, qRT-PCR evaluation was performed to figure out the mRNA expression of BAK1, BES1, BSU1, SPS, SBE, protochlorophyllide oxidoreductase (POR), DFR, CycD3, threonine synthase (TS), glutamine synthetase (GS), arginine decarboxylase (ACD), and inducer of C-repeat-binding issue expression (ICE) within the 5 samples (Fig. 3). The expression profiles on the single genes detected in qRT-PCR evaluation coincided with those detected inside the RNA-seq datasets.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes involved within the BR signal transduction pathwayKEGG enrichment annotation revealed that 26 genes are involved within the BR signal transduction pathway (Fig. 4: 1). KEGG analysis showed that compared with CAK (BR spraying for 0 h), the expression levels of BRI1, BAK1, transmembrane kinase 4 (TMK4), 14-3-3, abscisic acid G-protein coupled receptor (GPCR), BSU1, BES1, and BES1-interacting myc-like 2 (BIM2) that are related to BR signal transduction have been upregulated following BR spraying (for 3 h, 9 h, 24 h, and 48 h), but the highest gene expression levels varied among time points, which could be because of the unique sequences of signal transduction.Exogenous spraying of BR promotes cell division, theanine synthesis, and elevated expression of genes connected to cold resistance in tea leavesKEGG enrichment and annotation revealed that quite a few cyclin genes in tea leaves had been upregulated by BR spraying (Fig. 4: two). Furthermore, three genes for theanine synthesis and a single gene connected to cold resistance wer.