On Cinnamon) is usually a commonly located medicinal plant in Sri Lanka with proven antioxidant activity. It belongs towards the household Lauraceae and has been reported to possess lots of beneficial activities for example becoming an antioxidant, antimicrobial, anticancer, anti-inflammatory, antidiabetic, antimutagenic and as an anti-tyrosinase agent (Rao and Gan, 2014). It was previously reported that the bark extract of Ceylon cinnamon has numerous antioxidant compounds, which can effectively counteract with reactive oxygen species (ROS) for instance hydroxyl radicals, superoxide anions at the same time as other totally free radicals. Several in vitro studies reported the antioxidant impact of Cinnamomum zeylanicum Blume inside the current previous (Rao and Gan, 2014; Ghosh et al., 2015; Ranasinghe and Galappaththy, 2016; Premakumara and Abeysekera, 2020). The necessary oils obtained from the bark of Cinnamomum zeylanicum Blume and eugenol have shown quite strong antioxidant activities (Chericoni et al., 2005) and in vitro studies revealed that Cinnamomum bark extracts properly scavenged two,diphenyl-1-picrylhydrazyl (DPPH) radicals and two,20 -azino-bis(3-et hylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations (Ranasinghe et al., 2013). Despite the fact that it has currently been verified that Cinnamomum zeylanicum Blume has a considerable antioxidant activity, effect of its bark extract has never been investigated against doxorubicin induced cardiotoxicity in an animal model. Therefore, the objective of this study was to investigate the ameliorative effects of Cinnamomum zeylanicum Blume bark against doxorubicin induced cardiac injury by the attenuation of oxidative pressure and structural cardiomyocyte alterations in rats. two. Material and approaches two.1. Collection of Cinnamomum zeylanicum Blume bark The cultivated Cinnamomum zeylanicum Blume bark was collected and identified according to the descriptions provided by Jayaweera (1982). The species identification was confirmed by the curator with the National Herbarium, Royal Botanical Gardens, Peradeniya, Sri Lanka. A voucher specimen (2015/PG/VS/02) was deposited in the Division of Biochemistry, Faculty of Medicine, University of Ruhuna, Sri Lanka. 2.2. Standardization of plant material 2.two.1. Physicochemical analysis The bark components (cut into smaller pieces) of Cinnamomum were dried at 40 till a continual weight was reached and finely grounded. The powdered plant material was taken for the physicochemical evaluation. Tests for moisture content material, extractable matter and heavy metal evaluation had been PPARĪ³ supplier followed according to the WHO standards (1996). Microscopic analysis from the plant was carried out in accordance with the WHO (2011) suggestions on high-quality control and standardization of plant materials. 2.two.two. Phytochemical analysis Phytochemical screening of Cinnamomum zeylanicum bark was followed to determine medicinally active substances found in the plant. Plant material was dried at 40 for 3 days, ground coarsely, and extracted in IL-8 Accession distilled water or organic solvents in line with the approach used. The relevant extracts were subjected to qualitative phytochemical screening assays for the detection of anthracene glycosides, cyanogenic glycosides, cardenoloid glycosides, saponins, polyphenols, alkaloids, flavonoids, tannins, reducing sugars and proteins (Trease and Evans, 2009; Mushtaq et al., 2014; Yusuf et al., 2014). two.2.three. Total polyphenol content material and in vitro antioxidant activity of aqueous bark extract of Cinnamomum zeylanicum (ABEC) Continual weight in the Cinnamomum zeylanicum ba.