Towards the progression of CRPC, permitting prostate cancer cells to develop despite AR targeted therapy. Final results and Conclusion: Additional targeted research will provide a biological understanding around the part of EV within the AR signalling axis, enabling the design of novel EV primarily based therapeutics to target CRPC. Grant support: The US DoD PCRP Postdoctoral Training Award [W81XWH-12-1-0047] and Notion Development New Investigator Award (W81XWH-15-PCRP-IDA) for CS, the Movember Worldwide Action Strategy (GAP1) for PJR, CCN, CS. References 1. NOP Receptor/ORL1 list Soekamaji C et al., Oncotarget. 2016; doi: ten.18632/oncotarget.11111. [Epub ahead of print]. 2. Soekmadji et al., Cancers. 2013; five(four):1522544 three. Soekmadji and Nelson, Biomed. Res. Int. 2015; 2015: 454837.metastatic cancer cells could SNIPERs Compound induce malignant properties in the recipient cells. To address this query, internalisation (uptake kinetics, effect of cell cycle) and functional effects (proliferation and migration) of EVs derived from metastatic and key prostate cancer (PCa) cells and benign prostate cells were analysed. Methods: EVs were isolated from LNCaP, PC-3, RC92a/hTERT and PNT2 cells by differential centrifugation at 20,000g for microvesicles and 110,000g for exosomes. Size and morphology of EVs were characterised by transmission electron microscopy and nanoparticle tracking evaluation, plus the presence of CD9, CD63, and HSP70 was analysed by western blotting. EVs were labelled with fixable lipophilic dyes. EV uptake was determined by high content microscopy, flow cytometry, and confocal microscopy. Cell cycle, proliferation and migration have been analysed to evaluate the functional effects on the various EVs on recipient cells. Results: EVs derived from LNCaP and PC-3 cells of metastatic origin have been internalised by the recipient cells (PCa and benign) much more efficiently than the EVs derived from major cancer RC92a/hTERT cells or benign PNT2 prostate cells, as shown by flow cytometry and high content material microscopy. No differences were detected in the internalisation rate of microvesicles and exosomes. Additional evaluation of EV uptake and cell cycle revealed larger EV numbers within the G2/M cells than inside the G0/G1 or S cells, indicating that the cell cycle may well play a part in active EV uptake. Metastatic cell-derived EVs from PC-3 and LNCaP cells prompted more proliferative and migratory behaviour in the recipient cells (PCa and benign) when compared with the EVs derived from major cancer or benign cells. Conclusion: These final results show that the uptake and functional capacity of EVs is determined by the metastatic state from the parent cells, encouraging extra investigation into the EV-mediated mechanisms that market tumour spread and metastasis inside the tumour microenvironment.PS06.Glycosylation promotes azurocidin sorting into EVs in clear cell renal cell carcinoma cells Kentaro Jingushi1, Takuya Naito1, Motohide Uemura2, Koji Ueda3, Kazutoshi Fujita2, Norio Nonomura2 and Kazutake Tsujikawa1 Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan; 2Department of Urology, Osaka University, Graduate School of Medicine, Osaka, Japan; three Project for Personalised Cancer Medicine, Cancer Precision Medicine Centre, Japanese Foundation for Cancer Research, JapanPS06.Uptake and functionality of prostate cancer extracellular vesicles is dependent upon the metastatic stage on the parental cells Elisa L aro-Ib ez1, Maarit Neuvonen1,2, Maarit Takatalo1,2, Uma Thanigai Arasu3, Cristian Capasso4, J.