On NextSeq Higher Output single-end sequencing run. Outcomes: Administration of AFSC-EVs elevated terminal bud density and surface area of lung explants back to handle levels and promoted lung epithelial cell differentiation in lung organoids (increased SPC andPF12.10=OWP2.HIV-specific antibody-mediated targeting of ENV+ tissues by exosomes Zou Xue, Yuan M’eng, Zheng Nan and Wu Zhiwei Nanjing University, Nanjing, China (People’s Republic)Introduction: ART (Antiretroviral Therapy) can effectively suppress HIV replication inside the peripheral blood to an undetectable level. However, efforts to eradicate the latent virus in reservoirs remain a challenge and are a significant obstacle inside the remedy of HIV sufferers. Exosomes exhibit massive promise as an endogenous drug delivery nanosystem for delivering drugs to reservoir tissues provided their distinctive properties, which includes low immunogenicity, innate stability, high delivery efficiency and mainly importantly the capability to penetrate solid tissues due to their lipophilic properties. Solutions: Within this study, we engineered and expressed the ScFv of a high affinity HIV-specific monoclonal antibody, 10E8, on exosome surface. Exosomes from 293T cells were loaded with curcumin CD115/M-CSF R Proteins Biological Activity through saponin, with efficient up to 34 . 10E8ScFv-expressing exosomes (10E8-Exo) showed very efficient targeting of and curcumin delivery to CHO cell that expresses a trimeric gp140 on its surface (ENV+ cells) in vitro as demonstrated by confocal imaging and flow cytometry. We showed that 10E8-Exo could proficiently bind to CHO cell that expresses a trimeric gp140 on its surface. The exosomes loaded with curcumin, a chemical that was shown to kill HIV-infected cells, showed specific killing of the trimeric gp140-expressing CHO cells. In an NCG mouse model that was grafted using the tumourigenic gp140-CHO cells and created strong tissue tumours intravenously injected 10E8-Exo targeted the ENV-expressing tissues and delivered curcumin to CD233 Proteins Species induce a strong suppression with the ENV+ tumour growth having a low toxicity. Outcomes: Our outcomes demonstrated that engineered exosomes can provide anti-HIV agents to strong tissues byISEV2019 ABSTRACT BOOKspecifically targeting cells expressing viral ENV and induce cell killings. Summary/conclusion: It suggesting that such an strategy may be developed for eradicating virusinfected cells in tissue reservoir Funding: This study was supported by The National Key Research and Development Program of China(2016YFC1201000), Nature Science Foundation of Jiangsu Province (BY2015069-02) and National Nature Science Foundation of China (81672020). The funders had no part in study design, information collection and analysis, selection to publish, or preparation of the manuscript.JOURNAL OF EXTRACELLULAR VESICLESLate breaking- EVs and cancer Chairs: Sonia Melo; Golnaz Morad Location: Level 3, Hall A 15:306:LBF01.Exosomes from LNCaP cells promote the activity of osteoblasts by means of the transfer of mir-375 Yun Yea and Su-liang Liba Prostate Cancer, Xi’an, China (People’s Republic); bCancer, Xi’an, China (People’s Republic)for Cancer Research, Tokyo, Japan; cCancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Analysis, Tokyo, JapanIntroduction: Studies have shown that exosomes influence tumour metastasis, diagnosis and therapy. It has been found that exosomal miRNAs are closely linked towards the metastatic microenvironment. Nonetheless, the regulatory role of exosomes from prostate cancer (PCa) cells in.