F Nanotechnology Sophisticated Materials, Bar-Ilan University, CD223/LAG-3 Proteins Storage & Stability Israel, Ramat Gan, USA; cSchool of Neurobiology, Biochemistry and Biophysics, Life CEACAM1 Proteins Biological Activity sciences faculty, Tel Aviv University, Israel, Tel Aviv, Israel; dSacklar School of medicine, department of human genetics and biochemistry Tel Aviv University, Israel, Petah Tikva, Israel; eSacklar College of Medicine, Division of Human Genetics and Biochemistry Tel Aviv University, Israel, Petah Tikva, USA; fSagol College of neuroscience, Tel Aviv University, Israel. College of Neurobiology, Biochemistry and Biophysics, Life Sciences Faculty, Tel Aviv University, Israel, Tel Aviv, Israel; gSagol College of Neuroscience, Tel Aviv University,bISEV2019 ABSTRACT BOOKIsrael, Sacklar School of Medicine, Department of Human Genetics and Biochemistry Tel Aviv University, Israel, Tel Aviv, USAIntroduction: Although exosoemes have been discovered to cross the blood rain barrier, their migration and homing abilities inside the brain stay unstudied. We’ve got not too long ago developed a approach for longitudinal and quantitative in vivo neuroimaging of exosomes, according to the superior visualization abilities of CT, combined with gold nanoparticles as labelling agents. Right here, we applied this technique to track the migration and homing patterns of intranasally administrated exosomes derived from bone marrow mesenchymal stem cells (MSC-exo) in diverse brain pathologies, which includes stroke, autism, Parkinson’s disease and Alzheimer’s illness. We identified that MSC-exo especially targeted and accumulated in pathologically-relevant murine models brains regions up to 96 h post administration, although in healthier controls they evacuated. The neuroinflammatory signal in pathological brains was highly correlated with MSC-exo accumulation. Additionally, MSC-exo have been selectively uptaken by neuronal cells in the pathological regions. Procedures: Exosomes were extracted from human bone marrow mesenchymal stem cells. They had been loaded with glucose-conjugated gold nanoparticles and weregiven via intranasal administration to mice with diverse pathologies. All mice have been scanned with CT 1, 24 and 96 h post administration. In addition, using PKH26 MSC-exo have been labelled and were visualized with complete brain florescence. Results: Altogether, our Data suggests that MSC-exo present distinct neurodistribution which is pathologyspecific in every with the mice models visualized each in vivo and ex-vivo. In both the induced stroke and Parkinson’s models, the MSC-exo were visualized mainly inside the broken tissue (Striatum). In Alzheimer’s model, they were visualized mainly inside the hippocampus, and in the Autism mice model, they had been visualized each within the prefrontal cortex plus the cerebellum. Interestingly, in healthier mice the exosomes didn’t dwelling to any precise place along with the signal was lost 24 h post administration each in vivo and ex vivo. In the broken tissue, the MSC-exo have been found mostly inside the neurons and not in other cells. Summary/conclusion: Taken collectively, these findings can significantly market the application of exosomes for therapy and targeted drug delivery in different brain pathologies by way of intranasal administration.JOURNAL OF EXTRACELLULAR VESICLESSymposium Session 22: Novel Solutions of EV Evaluation Chairs: An Hendrix; John Nolan Location: Level B1, Hall A 16:308:OF22.Biolayer interferometry extracellular vesicles (BLIEV) platform for liquid biopsy of ovarian cancer Tatu Rojalina, Randy Carneya and Kit LambaUC Davis, Davis, USA; bUniversity of California,.