Ia P=0.30 20 10P=0.eight 6 four 2P=0.0.Haemophilus Veillonella Slackia RP101988 Technical Information Granulicatella AcidaminococcusRYGB-pre RYGB-postSG-preSG-postRYGB-pre RYGB-postSG-preSG-postAnaerostipesRelative
Ia P=0.30 20 10P=0.eight six four 2P=0.0.Haemophilus Veillonella Slackia Granulicatella AcidaminococcusRYGB-pre RYGB-postSG-preSG-postRYGB-pre RYGB-postSG-preSG-postAnaerostipesRelative abundance Blautia4 three two 1VeillonellaP=0.Clostridium Fusobacterium Oscillospira Collinsella RYGB SGRelative abundance Bifidobacterium P=0.P=0.RYGB-pre RYGB-postSG-preSG-postRYGB-pre RYGB-postSG-preSG-postFigure 1. (a) Heatmap of the alterations inside the relative abundance on the microbiota profile at Phylum, Household and Genus levels Figure 1. (a) Heatmap with the adjustments inside the relative abundance of the microbiota profile at Phylum, Loved ones and Genus oflevels with the two bariatric surgery procedures tested, Roux-en-Y gastric bypass and sleeve gastrectomy (SG). Differences the two bariatric surgery procedures tested, Roux-en-Y gastric bypass (RYGB) (RYGB) and sleeve gastrectomy (SG). Difinferences in percentage are shown. changes inside the RYGB procedure, indicates modifications amongst procedures. (b) Bar percentage are shown. Indicates Indicates modifications inside the RYGB procedure, indicates modifications involving proceplots with the statistically considerable bacteria in the preceding heatmap. dures. (b) Bar plots of your statistically substantial bacteria in the previous heatmap.2.4. Bile Acids Profile 2.4. Bile Acids Profile Normally terms, bile acids abundances were reduced using the two procedures of Generally terms, bile acids abundances had been reduced using the two procedures of bariatric surgery, principal and secondary bile acids. Although without having statistic signifibariatric surgery, main and secondary bile acids. Although devoid of aa statistic significance, RYGB suffered the highest reduction (Figure 2a). cance, RYGB suffered the highest reduction (Figure 2a). Deeping in to the alterations of key bile acids, fold changes are GLPG-3221 Biological Activity represented inside the heatmap of Figure 2b. No major bile acids adjustments were statistically considerable among procedures. Having said that, observing fold transform trends inside the figure, it might be inferred that there is a reduction within the the majority of the primary bile acids with RYGB, together with the exception of taurochenodeoxycholate and taurocholate. In SG, the key bile acids that seem to become enhanced were chenodeoxycholate, chenodeoxycholic sulfate 2 and cholate sulfate. Regarding the secondary bile acids, in a basic manner, had been reduced within the RYGB although enhanced in SG. The following secondary bile acids fold adjustments differed involving groups (p 0.05): deoxycholic_acid_12_or_24_sulfate, isoursodeoxycholate sulfate 1 and Lithocholate-sulfate1 and Lithocholic-acid-sulfate2, with all the subsequent ones showed a tendency (p 0.1): a3-dehydrocholate, a3-dehydrodeoxycholate, a7-ketolithocholate, and Ursodeoxycholate-sulfate1 (Figure 2c, Supplementary Figure S2).Metabolites 2021, 11, 733 Metabolites 2021, 11, x FOR PEER REVIEW5 of 14 5 ofa)Bile Acids2.0 1.five 1.0 0.five 0.c)RYGB SGSecondary Bile Acidsa12-dehydrocholate a12-ketolithocholate a3-b-hydroxy-5-cholenoic acid a3-dehydrocholate a3-dehydrodeoxycholate a6-oxolithocholate a7-ketodeoxycholate a7-ketolithocholate Dehydrolithocholate DeoxycholateFold ChangePrimary Bile Acids Secondary Bile AcidsDeoxycholic acid-12-or-24-sulfate Deoxycholic-acid-3-sulfateb)Key bile acidsChenodeoxycholate Chenodeoxycholic acid sulfateGlycocholenate-sulfate Glycodeoxycholate Glycolithocholate-sulfate Glycoursodeoxycholate Hyocholate Isoursodeoxycholate Isoursodeoxycholate-sulfate1 LithocholateChenodeoxycholicacid sulfate 2 CholateCholate sulfate G.