Fter TLC-DB plus the extraction parameters of SCF procedure. P. aeruginosa Sample 1 two three 4 5 six 7 8 9 10 11 12 13 14 15 16 17 18 19 Diameter (mm) five.86 five.50 7.51 6.18 five.43 0.00 4.29 4.83 0.00 six.50 six.91 six.27 five.41 5.71 0.00 6.37 6.56 0.00 5.MRSA SD Diameter (mm) five.46 4.85 7.57 four.69 4.80 0.00 four.66 four.32 0.00 4.59 four.77 3.29 three.41 four.25 0.00 3.50 3.29 0.00 four.SDMPa 20 15 10 20 15 ten 20 15 ten 20 15 10 20 15 10 20 15 10CEtOH two two two two 2 2 2 two two 1.five 1.five 1.five 1.5 1.5 1.five 1.5 1.5 1.50.72 0.56 0.85 0.64 0.42 0.00 0.71 0.29 0.00 0.42 0.20 0.33 0.47 0.80 0.00 0.78 0.55 0.00 0.0.50 0.78 0.62 0.46 0.38 0.00 0.39 0.59 0.00 0.65 0.69 0.42 0.36 0.35 0.00 0.40 0.46 0.00 0.40 40 40 60 60 60 80 80 80 40 40 40 60 60 60 80 80 80Molecules 2021, 26,4 ofTable 1. Cont. P. aeruginosa Sample 20 21 22 23 24 25 26 27 chloroform ch EtOH EtOH Vancomycin Gentamicin Diameter (mm) six.34 five.32 five.61 four.98 0.00 3.02 2.75 0.00 0.00 0.00 0.00 8.MRSA SD Diameter (mm) 3.58 three.72 three.72 3.07 0.00 three.40 three.25 0.00 0.00 0.00 0.00 12.30SDMPa 15 10 20 15 ten 20 15CEtOH 1 1 1 1 1 1 ten.82 0.41 0.47 0.61 0.00 0.23 0.48 0.00 0.00 0.00 0.00 0.0.82 0.40 0.40 0.36 0.00 0.58 0.68 0.00 0.00 0.00 0.00 0.45 -40 40 60 60 60 80 80SD = typical deviation; ch = chloroform.Figure 1. (a) Sutezolid Technical Information Detection in the antibacterial activity of clary sage SC-CO2 extracts, good handle (gentamicin), and solvents against P. aeruginosa by TLC-DB. The numbering of the samples will be the similar as that shown in Table 1; (b) Detection from the antibacterial activity of clary sage SC-CO2 extracts, constructive control (vancomycin), and solvents against MRSA by TLC-DB. The numbering on the samples is the very same as that shown in Table 1.Molecules 2021, 26,five ofFigure two. Antibacterial activity of each clary sage extracts and gentamicin as optimistic control on P. aeruginosa. The numbering of your samples is the same as that shown in Table 1.Figure 3. Antibacterial activity on MRSA of every clary sage extracts and vancomycin as optimistic handle. The numbering of the samples is the exact same as that shown in Table 1.Molecules 2021, 26,six of2.two. Response Surface Results The response surface models from the extracts have been plotted by using the Style Professional plan. Figures four and five show how the antibacterial activity of your extract alterations against bacterial strains of P. aeruginosa and MRSA as well as the continuous adjust of specific parameters. It can be observed that inside the case of P. aeruginosa, the diameter of the zones of inhibition was bigger on typical, so the extracts have been much more productive against this kind of bacterium. The shape from the response surfaces is quite related in the two Aztreonam site situations studied. Because the figures show, the best outcomes have been obtained at low temperatures (405 C) and moderately larger pressures (159 MPa). The least productive extracts is often prepared at 400 C and 101 MPa. Throughout the extraction, the plant material has to be protected from excessive heat. As a function of the cosolvent ratio, the shape on the figures is less characteristic, from which it may be concluded that of your three setting parameters, the EtOH concentration has the least impact around the antibacterial activity. Inside the case of P. aeruginosa, the optimal ratio may be between 1.6 and 1.7 , along with a further boost within the EtOH ratio will no longer generate a important improvement in antibacterial activity. However, within the case of MRSA, the most effective final results can be obtained using a cosolvent ratio of 2 . For each bacterial strains, the worst results were obtained at a ratio of 1.0.two EtOH.Figure four.