By the Russian Foundation of Science (# 19-14-00110). Institutional Critique Board Statement: Not applicable. Informed Consent Statement: Not applicable. Data Availability Statement: Not applicable. Conflicts of Interest: The authors declare that the research was carried out within the absence of any MRTX-1719 Epigenetic Reader Domain industrial or monetary relationships that might be construed as a potential conflict of interest.
H OH OHmetabolitesArticleDynamics of your Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature VariationsDamien Le Moigne 1 , Justine Demay 1,2 , Anita Reinhardt 2 , C ile Bernard 1 , Sandra Kim Tiam 1,3 and Benjamin Marie 1, UMR7245 Mol ules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Mus m National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, France; [email protected] (D.L.M.); [email protected] (J.D.); [email protected] (C.B.); [email protected] (S.K.T.) Thermes de Balaruc-Les-Bains, 1 Rue du Mont Saint-Clair BP 45, 34540 Balaruc-Les-Bains, France; [email protected] UMR5557 Laboratoire d’Ecologie Microbienne, Universitde Lyon, 43 bd du 11 novembre 1918, Villeurbanne, F-69622 Lyon, France Correspondence: [email protected]; Tel.: 33-1-4079-Citation: Le Moigne, D.; Demay, J.; Reinhardt, A.; Bernard, C.; Kim Tiam, S.; Marie, B. Dynamics from the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature Variations. Metabolites 2021, 11, 745. https://doi.org/ 10.3390/metabo11110745 Academic Editor: Daniel Globisch Received: 23 August 2021 Accepted: 26 October 2021 Published: 29 OctoberAbstract: Cyanobacteria are microorganisms able to adapt to a wide variety of environmental situations and abiotic stresses. They generate a large quantity of metabolites which will participate in the dynamic adaptation of cyanobacteria to a array of different light, temperature, and nutrient situations. Studying the metabolite profile is one particular approach to understand how the physiological status of cells is related to their adaptive response. Within this study, we sought to understand how the diversity and dynamics from the complete metabolome depended on the development phase and several abiotic variables which include light intensity and temperature. The cyanobacterium, Aliinostoc sp. PMC 882.14, was chosen for its big quantity of biosynthetic gene clusters. A single group of cells was grown beneath normal situations as a manage, when other groups were grown below Polmacoxib custom synthesis larger light or temperature. Metabolomes have been analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted evaluation to investigate metabolite dynamics, and significant variation was discovered among exponential and stationary phases, no matter culture circumstances. In the higher light group, the synthesis of a number of metabolites, like shinorine, was induced while other metabolites, such as microviridins, were synthesized below greater temperature conditions. Amongst highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the value of taking into consideration the physiological state of cyanobacteria for comparative global metabolomics and studies with the regulatory processes involved in production of particular metabolites. Our outcomes also open up new perspectives around the use of organisms for instance cyanobacteria for the targeted production of bioactive metabolites. Key phrases: cyanobacteria;.