Y debris, along with the chosen sperm additional showed linear progressive motilities with high speed. In addition, the amount of spermhead vacuoles and volume of sperm DNA fragmentation have been drastically decreased with each PVP media concentrations compared using the manage. Our simulation study also showed that the sperm in extremely viscous media exhibit highly linear motions due to the considerably reduced rotational motions. This directional motion with the sperm causes velocity-dependent translational diffusion, resulting in separation of motile sperms from raw semen containing motile and immotile sperm, distinctive blood cells, and debris. These results exemplify the filtration principle for sperm in the cervical canal, exactly where the viscous atmosphere with the cervical mucus filters out sperm with poor motility, as demonstrated by our SSC having a viscous PVP medium in vitro. Primarily based on these outcomes, we intend to carry out additional clinical research with human oocytes to examine the functionality of your sperm chosen by the proposed SSC. The PVP resolution has been usually applied for sperm immobilization through ICSI inside the clinics. Nevertheless, potential dangerous effects of PVP on sperm have already been reported by Kato et al. [41]. As a way to much more safely use SSC, it’s as a result essential to develop new supplies bio-compatible to sperm, oocyte, and embryo, which could replace the currently employed PVP inside the future. The presented SSC chip using a PVP medium could contribute to improving male fertility by way of high-quality sperm production as well as revealing the sperm navigation mechanisms within the female reproductive tract.Supplementary Supplies: The Supplementary Supplies are obtainable online at https://www.mdpi. com/article/10.3390/biomedicines9101439/s1. In Supplementary Materials (SM), we offer the two videos of experiments, (I) micro-viscometry utilizing our home-built setup and (II) sperm motion in three options with distinctive viscosities observed by optical microscope (Figure 4A), and (III) Table of the data of ten patients’ sperm made use of for our experiments. SM I (Video 1): Micro-viscometry of sperm sorting solutions. The video shows the quartz tuning fork with an optical fiber tip and theBiomedicines 2021, 9,12 ofmeasured answer, SAGE. We release a drop of sample fluid on mica substrate, the tip approaches the fluid sample until the tip’s bottom gently touches the sample surface, and then retracts away in the sample till the fluid bridge is ruptured, when measuring the shear interaction among the tip and the fluid. From the measured shear force, we calculate the viscosity on the sample fluid. SM II (Video 2): Sperm motion in Control (PVP 0 ), PVP 1.5 , and PVP 3.0 (Figure 4A in the primary text). SM III (Table S1): The facts of ten patients’ sperm data employed in our study. Author Contributions: J.K.C., J.H.L., J.J.K., J.W.P. and M.L. conceived and created the study. M.L., D.K., H.K., M.J.C., E.J.L., T.E.S., D.K.K., S.L. and D.G.B. carried out experiments. J.K.C., M.L. and J.H.L. analyzed and interpreted the information, and wrote the manuscript. All authors have read and agreed for the published version of the manuscript. Funding: This analysis was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT) (NRF-2017R1C1B5076000, NRF-2017R1C1B5076655, SB-612111 MedChemExpress NRF2020R1F1A1073628, 2021 R D Equipment Engineer Education Program-2018R1A6A9056986), the basic Science Analysis Program by means of the NRF (2019R1A2C1086882), Medi-Chal.