D they’re vital contributors towards the flavor of tea.We
D they’re important contributors towards the flavor of tea.We focused our analyses around the biosynthetic pathways of those metabolites and the differential expressions of the associated genes inside the C.sinensis tissues.According to the KEGG database, a total of unigenes were annotated and found to be connected together with the biosynthetic pathways in the three metabolites.Table Summary of unigene annotationsDatabase Total Annotated unigenes unigenes Nr SwissProt CDD TrEMBL TAIR Total , , , , , , , , , , , , Percent ……Nonannotated % unigenes , , , , , , ……Flavonoids are a group of plant polyphenol secondary metabolites that involves flavones, flavonols, isoflavones, flavanones, flavanols, and anthocyanidins.The flavanols, or catechins, are the most prominent flavonoid compounds in leaves .These compounds contribute to numerous of your attributes that PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21330814 make tea a extremely worthwhile component of your human diet.The central pathways for flavonoid biosynthesis are highly conserved and well characterized .Flavonoids are synthesized in the basic phenylpropanoid pathway.Inside the flavonoid pathway, chalcone synthase (CHS) catalyzes the initial step within the biosynthesis of flavonoids.Flavanone hydroxylase (FH) catalyzes the formation of dihydroflavonols from flavanones (Fig.a).We located 5 unigenes annotated as FH according to the KEGG pathway.Among them, three unigenes (c c and c) were globally expressed in all the tissues (Fig.b).With the other two unigenes, a single (c) was expressed in the majority of the tissues, but not in old leaves, and the other (c) was only expressed in second leaves.The three FH unigenes (c c and c) could be transcribed in the same gene by alternative splicing.The dihydroflavonols serve as intermediates for the biosynthesis of flavonols, flavanols, and anthocyanidins .In tea, the flavonoid pathway has been implicated in the biosynthesis of catechins .Leucoanthocyanidins will be the direct precursors of flavanLi et al.BMC Genomics Page ofFig.Functional classifications of unigenes from C.sinensis.a COG (cluster of orthologous groups) classifications with the unigenes.A RNA processing and modification; B Chromatin structure and dynamics; C Power production and conversion; D Cell cycle control, cell division, chromosome partitioning; E Amino acid transport and metabolism; F Nucleotide transport and metabolism; G Carbohydrate transport and metabolism; H Coenzyme transport and metabolism; I Lipid transport and metabolism; J Translation, ribosomal structure and biogenesis; K Transcription; L Replication, recombination and repair; M Cell wallmembraneenvelope biogenesis; N Cell motility; O Posttranslational modification, protein turnover, chaperones; P Inorganic ion transport and metabolism; Q Secondary metabolites biosynthesis, transport and catabolism; R Common FCCP MedChemExpress function prediction only; S Function unknown; T Signal transduction mechanisms; U Intracellular trafficking, secretion, and vesicular transport; V Defense mechanisms; Y Nuclear structure; Z Cytoskeleton; b Gene ontology (GO) classifications of unigenes in tissues.The 3 pie charts show the significant categories of GO terms, that are Cellular Component (CC), Biological Approach (BP), and Molecular Function (MF).Every single main category was additional divided into numerous subcategories whose proportions are shown underneath the labelsols (e.g catechin and gallocatechin) produced by leucoanthocyanidin reductase (LAR).There had been six LAR unigenes (c c c c c c) in our database.One unigene (c) was particularly expre.