Mouse anti-Tubulin α Monoclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin. |Host Mouse |Specificity The α-tubulin antibody can detects ebrafish endogenousα-tubulin protein. |Species Reactivity Zebrafish |Applications WB: 1:3000~1:10000, ELISA(peptide) 1:20000-1:40000 |Immunogen Peptide from Zebrafish alpha tubulin. |Properties |Concentration 1mg/ml |Purification affinity purification |Clonality Monoclonal Antibody |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody. |Target |Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |Post-translational modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. |Cellular localization Cytoskeleton;Cytosol;Extracellular region or secreted; |UniProt P68366 | |Tips:This product is for research use only. Not for use in diagnostic prodcedures.